In TRA that seek to preserve sperm quality, GSH has been used as an antioxidant, trying to extend the lifespan of the male gamete. Although ROS cause cell damage, it is also clear that they play an important physiological role in sperm during SC which in turn affects fertilization. Therefore...
Introducción: La mayoría de los estudios cuyo objetivo es evaluar las tasas de recuperación y desarrollo post-vitrificación de ovocitos porcinos han reportado mejores tasas cuando estos son criopreservados en estados embrionarios, pero no así en ovocitos inmaduros. Por esta razón, para mejorar los protocolos de la vitrificación de ovocitos inmaduros,...
Obesity is related to reproductive problems such as: higher amount of apoptotic oocytes, a lower number of recovered oocytes, oocytes of smaller size and lower number of mature oocytes. Therefore, women with obesity are candidates for assisted reproductivetechniques. However, these women require higher doses of gonadotropins to achieve ovarian stimulation...
This study was designed to evaluate the efficiency of bovine embryo vitrification in the morula stage, using Ethylene Glycol (EG) and trehalose in the beveled straw (beveled edge straw; BES) and its effects in viability and development potential after devitrification. For embryo production in vitro (IVP) ovaries were collected at...
La vitrificación es una técnica de criopreservación empleada principalmente en gametos. Esta técnica tiene como finalidad mantener la viabilidad celular, su funcionalidad y su potencial de desarrollo a -196°C. Durante este proceso se requiere de la adición de agentes crioprotectores (CPAs), que son sustancias que confieren protección a las células...
La primera etapa del embarazo humano y la preñez murina se caracteriza por ser pro- inflamatoria, durante ésta se da una comunicación entre el embrión y el útero mediante señales moleculares, que pueden determinar el rechazo o la aceptación del embrión por el endometrio uterino. Una de las primeras señales...
Intracytoplasmic sperm injection (ICSI) success is restricted by failure in oocyte activation and in sperm head decondensation with low male pronuclei (PN) formation. In order to improve ICSI, appropriate sperm selection and oocyte activation are necessary. The objective of the present study was to determine the efficiency of fertilization using...
Malathion is one of the most commonly used insecticides, recent findings have demonstrated that it induces oxidative stress in somatic cells, but there are not enough studies demonstrating this effect in germ cells. Malathion impairs porcine oocyte viability and maturation, but studies have not shown how oxidative stress affects maturation...