The present work was focused on the characterization of Aspergillus brasiliensis proteome, assessing the culture system and sucrose concentration present in the culture medium. The work was parted into two stages. In the first stage, the effect of sucrose concentration under SSF (20 to 210 g/L, using perlite as support)...
Las amilasas son enzimas de importancia industrial que llevan a cabo la hidrólisis del almidón. Las amilasas producidas por cultivo sumergido (CS) están sujetas a la represión catabólica por carbono en presencia de glucosa en el medio de cultivo. En cambio, el cultivo en medio sólido (CMS) ha mostrado ventajas...
The present study compared the A. brasiliensis ATCC 9642 secretome obtained by submerged fermentation using the minimal medium Hill y Kafer with three initial concentrations of sucrose: 20, 40 and 60 g/L. The samples for the analysis of secretomes were taken at the culture time where the maximum rate of...